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Expansion and Differentiation of H7 Human Embryonic Stem Cells in Chemically Defined Medium Using Corning® Synthemax™ Surface
21 Jun 2010: In this application note Corning Synthemax Surface is used to support long-term self-renewal and differentiation of H7 hESCs. Cells demonstrated a stable doubling time, high level of expression of hESC phenotypic markers (Oct4, SSEA4 and TRA-1-60) and normal karyotype. Corning Synthemax Surface also supported successful directed differentiation of H7 cells into functional cardiomyocytes, proving suitability of Corning Synthemax Surface for both expansion and differentiation of hESC culture.
Corning Life Sciences
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Human Oncostatin M is Functionally Equivalent to Mouse LIF in Supporting Mouse ES Cell Pluripotency & Germline Competency
21 Jun 2010: In this application note R&D Systems demonstrate human OSM is able to maintain ES cell pluripotency in a similar manner to mouse LIF, including long-term maintenance of pluripotency and the ability to differentiate in vitro, the ability to activate STAT3, and germline transmission competency. The study suggests that OSM may be an additional factor that plays a role in early embryonic development and that it may be used as an alternative to LIF in mouse ES cell culture.
R&D Systems, Inc.
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CO2 Incubator Sterilization method with 90ºC Moist Heat Decontamination
17 Jun 2010: In this application note, Esco validate the Swiftcon 90ºC moist heat decontamination cycle of the Celculture CO2 Incubator by performing decontamination tests on 5 bacterial suspension preparations, including Aspergillus niger and Bacillus subtilis. After decontamination, the microbial suspension discs were placed on tubes containing PBS which were assayed by culturing methods to quantitate Total Viable Count at each location.
Esco Micro Pte Ltd
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Stem Cell-Derived Human Neurons in CNS Drug Discovery
07 Jun 2010: In this application note from Molecular Devices reproducible batches of human neurons are generated that will significantly improve and impact CNS drug discovery. The stem cell-derived neurons express all known markers typically associated with mature neurons and are functionally active. These neurons have been used in a recent screen to identify antagonists of a novel GPCR target implicated in schizophrenia.
Molecular Devices
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Corning® Epic® System: Real-time Monitoring of Cell Response to Virus Infection
24 May 2010: This application note discusses the use of the Epic System, an optical sensor-based label-free HTS platform (1), to monitor real-time the HeLa cell response to human rhinovirus (HRV) infection.
Corning Life Sciences
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Optimizing Donor Number for Consistent Pooled Human Liver Microsomes
08 Apr 2010: Human liver microsomes (HLMs) are commonly used as an in vitro reagent for the study of human CYP in the metabolism of a drug candidate. This application note presents an analysis of the CYP activity distributions for over 300 characterized (HLM) samples and predicts the variability in pooled CYP activity as a function of donor number. An optimal donor number based on observed variability can be selected.
BD Biosciences
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HTRF® Solutions for Oncology
04 Feb 2010: This application note presents a comprehensive line of HTRF (homogeneous time-resolved fluorescence) reagents for assessing oncology-related targets such kinases, ATPases, ubiquitin and heparanase assays. In addition, HTRF reagents are available for studying cell signaling, biomarker discovery, ligand binding, receptor and second messenger.
Cisbio Bioassays
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A Flow Cytometer with Fixed Voltage PMTs and Expanded Dynamic Range Simplifies Collection and Analysis of Complex Cell Populations
13 Mar 2009: Due to the wide variations in fluorescence and light scatter signals produced by different types of cells and particles, flow cytometrists often spend significant time, and waste significant amounts of sample, optimizing amplifier gains and/or PMT voltage settings. We demonstrate here that a flow cytometer equipped with fixed voltage PMTs calibrated to operate in linear range, can be used to analyze a wide variety of sample sizes.
Accuri Cytometers, Inc.
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AFP Assay on Olympus AU3000i Immunoassay Analyser
 08 Jul 2008: The AU3000i is a new immunoassay analyser from Olympus Diagnostics. It is a high throughput analyser, using the same sample racks as the Olympus family of general chemistry analysers (AU640, 2700, etc). This poster presents an assessment of the AFP assay as conducted in a District General Hospital in the North East of England
Olympus Europa Holding GmbH
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blood transfusion
